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KMID : 0616120160470030197
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Korean Journal of Pharmacognosy
2016 Volume.47 No. 3 p.197 ~ p.203
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Decursin from Angelica gigas Nakai Promotes Cytotoxicity and Induces Apoptosis in THP-1 cells, a Human Acute Monocytic Leukemia
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Kim Nam-Seok
Jeong Seung-Il
Kim Jong-Seok
Oh Mi-Jin
Oh Chan-Ho
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Abstract
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Decursin is a major component of the root of Angelica gigas(Umbelliferae), which has been traditionally used in Korea as a tonic and to treat anemia, hemiplegia, and women’s diseases. The objective of this study is to identify the anti-cancer mechanism induced by decursin on apoptosis of human leukemia and lymphoma cells. Cytotoxicity of decursin on U937, HL-60, MOLT-4, THP-1 cells showed the significant effects. First of all, IC50 of decursin on four cell lines was 27.1, 32.4, 17.4, 15.1 mM, respectively. So IC50 in THP-1 cells was the smallest among 4 cell lines treated with decursin(15.1 mM). In order to understand the apoptosis-mechanism by decursin, we examined the gene expression of bcl-2(anti-apoptotic), bax(pro-apoptotic)and p53(tumor suppressor)after treating the THP-1 cells with decursin(10, 50 and 100 mM). It was found bcl-2 gene was decreased dose dependently, the expression level of bax gene of THP-1 cells treated with 100 mM of decursin was about 3 times higher than those of control, and p53 gene was increased In the same concentration(100 mM), p53 gene was increased dose dependent manner. In protein express, bcl-2 and p53 protein showed a tendency to decrease. bax was increased about 4 fold. Therefore decursin is a useful chemotherapeutic agent against leukemia.
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KEYWORD
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Decursin, Apoptosis, Bcl family, p53, THP-1 cells
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